Symbiont-mediated RNA interference in insects

Whitten, Miranda M. A., Facey, Paul D., Del Sol, Ricardo, Fernández-Martínez, Lorena T., Evans, Meirwyn C., Mitchell, Jacob J., Bodger, Owen G. and Dyson, Paul J. (2016) Symbiont-mediated RNA interference in insects. Proceedings of the Royal Society B: Biological Sciences, 283 (1825). p. 20160042. ISSN 0962-8452 DOI

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RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides.We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects.

Item Type: Article
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Biology
Date Deposited: 21 Mar 2016 15:44

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