A transposon insertion single-gene knockout library and new ordered cosmid library for the model organism Streptomyces coelicolor A3(2)

Fernández-Martínez, L. T., Del Sol, R., Evans, M. C., Fielding, S., Herron, P. R., Chandra, G. and Dyson, P. J. (2011) A transposon insertion single-gene knockout library and new ordered cosmid library for the model organism Streptomyces coelicolor A3(2). Antonie van Leeuwenhoek, 99 (3). pp. 515-522. ISSN 0003-6072 DOI https://doi.org/10.1007/s10482-010-9518-1

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Abstract

A simple and high-throughput transposon mediated mutagenesis system employing in vitro shuttle transposon mutagenesis has been used to systematically mutagenise the Streptomyces coelicolor genome. To achieve the highest coverage, a new ordered cosmid library was also constructed. Individual cosmids from both the existing and new libraries were disrupted using the Tn5-based mini-transposon Tn5062. A total of 35,358 insertions were sequenced resulting in the disruption of 6,482 genes (83% of the predicted open reading frames). Complete information for both the newly generated cosmids as well as all the insertions has been uploaded onto a central database, StrepDB (http://​strepdb.​streptomyces.​org.​uk/​). All insertions, new cosmids and a range of transposon exchange cassettes are available for study of individual gene function.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Biology
Date Deposited: 22 Mar 2016 10:49
URI: http://repository.edgehill.ac.uk/id/eprint/7324

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