The Hsp72 and Hsp90α mRNA responses to hot downhill running are reduced following a prior bout of hot downhill running, and occur concurrently within leukocytes and the vastus lateralis

Tuttle, James, Christmas, Bryna Catherine Rose, Gibson, Oliver R, Barrington, James H, Hughes, David C, Castle, Paul C, Metcalfe, Alan, Midgley, Adrian, Pearce, Oliver, Chindu, Kabir, Rayanmarakar, Faizal, Al-Ali, Sami, Lewis, Mark P and Taylor, Lee (2017) The Hsp72 and Hsp90α mRNA responses to hot downhill running are reduced following a prior bout of hot downhill running, and occur concurrently within leukocytes and the vastus lateralis. Frontiers in Physiology, 8 (473). pp. 1-15. ISSN 1664-042X DOI https://doi.org/10.3389/fphys.2017.00473

This is the latest version of this item.

[img]
Preview
Text
Tuttle et al ACCEPTED VERSION.pdf - Accepted Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
[img]
Preview
Text
Tuttle et al 2017 (The Hsp72 & Hsp90 mRNA responses).pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

The leukocyte heat shock response (HSR) is used to determine individual’s thermotolerance. The HSR and thermotolerance are enhanced following interventions such as preconditioning and/or acclimation/acclimatisation. However, it is unclear whether the leukocyte HSR is an appropriate surrogate for the HSR in other tissues implicated within the pathophysiology of exertional heat illnesses (eg skeletal muscle), and whether an acute preconditioning strateg (eg downhill running) can improve subsequent thermotolerance. Physically active, non-heat acclimated participants were split into two groups to investigate the benefits of hot downhill running as preconditioning strategy. A hot preconditioning group (HPG; n = 6) completed two trials (HPGHOTDOWN1 and HPGHOTDOWN2) of 30 min running at lactate threshold (LT) on -10 % gradient in 30°C and 50 % relative humidity (RH) separated by 7 d. A temperate preconditioning group (TPG; n = 5) completed 30 min running at LT on a -1 % gradient in 20°C and 50 % (TPGTEMPFLAT) and 7 d later completed 30 min running at LT on -10 % gradient in 30°C and 50 % RH (TPGHOTDOWN). Venous blood samples and muscle biopsies (vastus lateralis; VL) were obtained before, immediately after, 3, 24 and 48 hr after each trial. Leukocyte and VL Hsp72, Hsp90α and Grp78 mRNA relative expression was determined via RT‐QPCR. Attenuated leukocyte and VL Hsp72 (2.8 to 1.8 fold and 5.9 to 2.4 fold; p < 0.05) and Hsp90α mRNA (2.9 to 2.4 fold and 5.2 to 2.4 fold; p < 0.05) responses accompanied reductions (p < 0.05) in 3 / 7 physiological strain [exercising rectal temperature (-0.3°C) and perceived muscle soreness (~‐14%)] during HPGHOTDOWN2 compared to HPGHOTDOWN1 (i.e. a preconditioning effect). Both VL and leukocyte Hsp72 and Hsp90α mRNA increased (p < 0.05) simultaneously following downhill runs and demonstrated a strong relationship (p < 0.01) of similar magnitudes with one another. Hot downhill running is an effective preconditioning strategy which ameliorates physiological strain, soreness and Hsp72 and Hsp90α mRNA responses to a subsequent bout. Leukocyte and VL analyses are appropriate tissues to infer the extent to which the HSR has been augmented.

Item Type: Article
Uncontrolled Keywords: Downhill running, heat shock response, heat stress, heat tolerance, preconditioning, cross tolerance, thermotolerance
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sports Science
Date Deposited: 22 Aug 2017 11:20
URI: http://repository.edgehill.ac.uk/id/eprint/9397

Available Versions of this Item

Archive staff only

Item control page Item control page