Bioavailability and Urinary Excretion of Phenolic-Derived Metabolites after Acute Consumption of Purple Majesty Potato in Humans

Tsang, Catherine, Smail, Nacer Foudil, MacDougall, Gordon J, Moosawi, Suzana AI and Dujaili, Emad AI (2015) Bioavailability and Urinary Excretion of Phenolic-Derived Metabolites after Acute Consumption of Purple Majesty Potato in Humans. EC Nutrition, 1 (3). pp. 96-105.

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Abstract

A novel purple potato variety, Purple Majesty (PM) contains an abundance of phenolic compounds, especially anthocyanins. The aim of this study was to assess the bioavailability of phenolic compounds in plasma measured as total polyphenols and urinary excretion of phenolic-derived metabolites after acute consumption of cooked PM. Five healthy male subjects (27-60 years; mean BMI: 26.7 ± 4.1) participated in a bioavailability study. Blood and urine were sampled at baseline and following consumption of 400 g cooked PM at 1h, 2h, 4h and 24h. A peak plasma antioxidant capacity was reached 1-2 hours post-consumption (from 1044 ± 281 µmol/L Fe(II) at baseline and increased to 1257 ± 180 after 1 hour (p = 0.045) and 1112 ± 251 µmol/L Fe(II) after 2 hours (p=0.06). Total phenols level in plasma was reached after 2 hours (from 342.4 ± 28.3 at baseline to 368.4 ± 25 umol/L GAE). Liquid chromatography mass spectrometric (LC-MS) analysis was used to track the levels of anthocyanin-like derivatives and metabolites in the urine of volunteers after intake of the cooked Purple Majesty potatoes. No anthocyanin derivatives were detected in urine by liquid chromatography mass spectrometry indicating levels were < 2 nM. The majority of peaks that increased after intake were putatively identified as sulphated phenolic metabolites. Phenolic glucuronides were identified but other peaks remain unidentified. Hippuric acid was identified as a major phenolic derivative. Hydroxy benzoic derivatives, characteristic of intake of anthocyanins, were not detected in urine, however metabolites expected from the B-ring of petunidin (i.e. methyl gallic acid) may have been obscured by other peaks. Some metabolites could have arisen through metabolism of chlorogenic acid, which is present at ~ equivalent amounts to anthocyanins in cooked PM. In conclusion, acute consumption of PM resulted in an increase in excretion of urinary phenolic derived metabolites. Identifying these unknown phenolic derivatives warrants further investigation.

Item Type: Article
Uncontrolled Keywords: Bioavailability; Purple Majesty potato; Urinary excretion; Anthocyanins
Subjects: R Medicine > R Medicine (General)
Divisions: Nursing and Midwifery
Date Deposited: 21 Sep 2017 15:17
URI: http://repository.edgehill.ac.uk/id/eprint/9578

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