The Fanconi anemia proteins FANCD2 and FANCJ interact and regulate each other's chromatin localization.

Chen, X, Wilson, J, McChesney, P, Williams, SA, Kwon, Y, Longerich, S, Marriott, Andrew, Sung, P, Jones, NJ and Kupfer, GM (2014) The Fanconi anemia proteins FANCD2 and FANCJ interact and regulate each other's chromatin localization. The Journal of Biological Chemistry, 289 (37). pp. 25774-25782. ISSN 1083-351X DOI https://doi.org/10.1074/jbc.M114.552570

[img]
Preview
Text
Chen 2014.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB) | Preview

Abstract

Fanconi anemia is a genetic disease resulting in bone marrow failure, birth defects, and cancer that is thought to encompass a defect in maintenance of genomic stability. Mutations in 16 genes (FANCA, B, C, D1, D2, E, F, G, I, J, L, M, N, O, P, and Q) have been identified in patients, with the Fanconi anemia subtype J (FA-J) resulting from homozygous mutations in the FANCJ gene. Here, we describe the direct interaction of FANCD2 with FANCJ. We demonstrate the interaction of FANCD2 and FANCJ in vivo and in vitro by immunoprecipitation in crude cell lysates and from fractions after gel filtration and with baculovirally expressed proteins. Mutation of the monoubiquitination site of FANCD2 (K561R) preserves interaction with FANCJ constitutively in a manner that impedes proper chromatin localization of FANCJ. FANCJ is necessary for FANCD2 chromatin loading and focus formation in response to mitomycin C treatment. Our results suggest not only that FANCD2 regulates FANCJ chromatin localization but also that FANCJ is necessary for efficient loading of FANCD2 onto chromatin following DNA damage caused by mitomycin C treatment.

Item Type: Article
Subjects: Q Science > QR Microbiology > QR180 Immunology
Divisions: Biology
Date Deposited: 19 Oct 2017 08:42
URI: http://repository.edgehill.ac.uk/id/eprint/9710

Archive staff only

Item control page Item control page