Reproducibility of measurement techniques used for creatine kinase, interleukin-6 and high-sensitivity C-reactive protein determination over a 48 h period in males and females

Chrismas, Bryna, Taylor, Lee, Smith, Alexander, Pemberton, Philip, Siegler, Jason Charles and Midgley, Adrian (2017) Reproducibility of measurement techniques used for creatine kinase, interleukin-6 and high-sensitivity C-reactive protein determination over a 48 h period in males and females. Measurement in Physical Education and Exercise Science. pp. 1-9. ISSN 1532-7841 DOI https://doi.org/10.1080/1091367X.2017.1412317

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Abstract

To examine the reproducibility of three measurement techniques used to determine creatine kinase, interleukin-6 and high-sensitivity C-reactive protein, 50 participants had blood samples taken on two occasions. Fingertip plasma samples were analysed using the Reflotron for CK determination. Venous blood samples collected into serum separator tubes were used for IL-6 and hs-CRP analyses. IL-6 was measured using an enzyme linked immune assay development kit. The hs-CRP was measured by an in-house ELISA method. Dependent t-tests showed no systematic bias between samples. The interdian CV was 20.0% for CK, 15.3% for IL-6 and 44.2% for hs-CRP. The intraclass correlation coefficient was 0.90 for CK, 0.98 for IL-6 and 0.70 for hs-CRP. The 95% limits of agreement were −69.7 to 63.5 IU/L for CK, −1.48 to 1.80 pg/ml for IL-6 and −1.10 to 0.91 μg/L for hs-CRP. The results demonstrate low absolute reproducibility, which may obscure a true experimental effect.

Item Type: Article
Uncontrolled Keywords: Exercise induced muscle damage; reliability; biomarkers; cytokines; inflammation
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sports Science
Date Deposited: 20 Dec 2017 15:57
URI: http://repository.edgehill.ac.uk/id/eprint/9920

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